Gel-free species identification using melt-curve analysis

DNA-based identification of organisms is an important tool in biosecurity, ecological monitoring and wildlife forensics. Current methods usually involve post-polymerase chain reaction (PCR) manipulations (e.g. restriction digest, gel electrophoresis), which add to the expense and time required for processing samples, and may introduce error. We developed a method of species identification that uses species-specific primers and melt-curve analysis, and avoids post-PCR manipulation of samples. The method was highly accurate when trialled on DNA from six large carnivore species from Tasmania, Australia. Because of its flexibility and cost-effectiveness, this method should find wide use in many areas of applied biological science.

Author OLIVER BERRY, STEPHEN D. SARRE
Date null
Year 2007
Secondary title Molecular Ecology Notes
Volume 7
Number 1
Institution Invasive Animals Cooperative Research Centre; Scho
Pages 1-4
Notes Notes
Region TAS
Links https://www.ingentaconnect.com/content/bsc/men/2007/00000007/00000001/art00001
https://www.blackwell-synergy.com/doi/abs/10.1111/j.1471-8286.2006.01541.x